Recombinant:Article Title: Transformation of an early-established motor circuit during maturation in zebrafish.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-GFP Abcam Cat# ab13970, RRID:AB_300798 Rabbit anti-Cx35.5 clone 12H5 Fred Hutch Antibody Technology Facility (Miller et al., 2017) N/A Mouse IgG2A anti-Cx34.1 clone 5C10A Fred Hutch Antibody Technology Facility (Miller et al., 2017) N/A Secondary antibody anti-chicken Alexa Fluor 488 Invitrogen Cat# A11039, RRID:AB_2534096 Secondary antibody anti-rabbit Alexa Fluor 568 Invitrogen Cat# A10042, RRID:AB_2534017 Secondary antibody anti-mouse Alexa Fluor 647 Invitrogen Cat# A31571, RRID:AB_162542 Streptavidin, Alexa Fluor 647 conjugate Invitrogen Cat# S32357 Streptavidin, Alexa Fluor 405 conjugate Invitrogen Cat# S32351 Chemicals, peptides, and recombinant proteins Ethyl 3-aminobenzoate methanesulfonate (MS-222) Sigma-Aldrich Cat# E10521 Neurobiotin Vector Laboratories Cat# SP-1120 PBS – Phosphate-Buffered Saline (10X) pH 7.4 Invitrogen Cat# AM9625 Triton X-100 Sigma-Aldrich Cat# T9284 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Tetramethylrhodamine-dextran Thermo Fisher Cat# D3308 Alexa Fluor 647-dextran Thermo Fisher Cat# D22914 D-(-)-2-amino-5-phosphonoic acid (AP-5) Tocris Cat# 0106/1 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f] quinoxaline (NBQX) Tocris Cat# 1044/1 Cadmium Sigma-Aldrich Cat# 202908 Experimental models: Organisms/strains Danio rerio: Tg(Chx10:GFP) (Kimura et al., 2006) ZFIN: ZDB-GENO-010924-10 Danio rerio: Tg(Chx10-loxP-dsRed-loxP-GFP) (Kimura et al., 2006) ZFIN: ZDB-TGCONSTRCT-070117-143 Danio rerio: Tol-056:GFP (Satou et al., 2009) ZFIN: ZDB-FISH-150901-13314 Danio rerio: Tg(VGlut2a-loxP-dsRed-loxPGal4) (Satou et al., 2013) ZFIN: ZDB-TGCONSTRCT-131127-2 Danio rerio: Tg(elavl3:cre) (Förster et al., 2017) ZFIN: ZDB-TGCONSTRCT-170921-3 Danio rerio: Tg(UAS:ChR2-YFP) (Fidelin et al., 2015) ZFIN: ZDB-TGCONSTRCT-150324-2 Software and algorithms pClamp Molecular Devices RRID: SCR_011323; https://www. moleculardevices.com/ Spike2 Cambridge Electronic Design RRID: SCR_000903; https://ced.co.uk MATLAB The MathWorks, Inc., Natick, Massachusetts, United States. .. RRID: SCR_001622; https://www.mathworks. com ImageJ (Schneider et al., 2012) RRID: SCR_003070; https://imagej.nih.gov/ij/ Adobe Photoshop Adobe Systems Inc., San Jose, CA RRID:SCR_014199; https://www.adobe.com/ products/photoshop.html Adobe Illustrator Adobe Systems Inc., San Jose, CA RRID:SCR_010279; http://www.adobe.com/ products/illustrator.html (Continued on next page) Cell Reports 39, 110654, April 12, 2022 e1
Saline:Article Title: Transformation of an early-established motor circuit during maturation in zebrafish.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-GFP Abcam Cat# ab13970, RRID:AB_300798 Rabbit anti-Cx35.5 clone 12H5 Fred Hutch Antibody Technology Facility (Miller et al., 2017) N/A Mouse IgG2A anti-Cx34.1 clone 5C10A Fred Hutch Antibody Technology Facility (Miller et al., 2017) N/A Secondary antibody anti-chicken Alexa Fluor 488 Invitrogen Cat# A11039, RRID:AB_2534096 Secondary antibody anti-rabbit Alexa Fluor 568 Invitrogen Cat# A10042, RRID:AB_2534017 Secondary antibody anti-mouse Alexa Fluor 647 Invitrogen Cat# A31571, RRID:AB_162542 Streptavidin, Alexa Fluor 647 conjugate Invitrogen Cat# S32357 Streptavidin, Alexa Fluor 405 conjugate Invitrogen Cat# S32351 Chemicals, peptides, and recombinant proteins Ethyl 3-aminobenzoate methanesulfonate (MS-222) Sigma-Aldrich Cat# E10521 Neurobiotin Vector Laboratories Cat# SP-1120 PBS – Phosphate-Buffered Saline (10X) pH 7.4 Invitrogen Cat# AM9625 Triton X-100 Sigma-Aldrich Cat# T9284 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Tetramethylrhodamine-dextran Thermo Fisher Cat# D3308 Alexa Fluor 647-dextran Thermo Fisher Cat# D22914 D-(-)-2-amino-5-phosphonoic acid (AP-5) Tocris Cat# 0106/1 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f] quinoxaline (NBQX) Tocris Cat# 1044/1 Cadmium Sigma-Aldrich Cat# 202908 Experimental models: Organisms/strains Danio rerio: Tg(Chx10:GFP) (Kimura et al., 2006) ZFIN: ZDB-GENO-010924-10 Danio rerio: Tg(Chx10-loxP-dsRed-loxP-GFP) (Kimura et al., 2006) ZFIN: ZDB-TGCONSTRCT-070117-143 Danio rerio: Tol-056:GFP (Satou et al., 2009) ZFIN: ZDB-FISH-150901-13314 Danio rerio: Tg(VGlut2a-loxP-dsRed-loxPGal4) (Satou et al., 2013) ZFIN: ZDB-TGCONSTRCT-131127-2 Danio rerio: Tg(elavl3:cre) (Förster et al., 2017) ZFIN: ZDB-TGCONSTRCT-170921-3 Danio rerio: Tg(UAS:ChR2-YFP) (Fidelin et al., 2015) ZFIN: ZDB-TGCONSTRCT-150324-2 Software and algorithms pClamp Molecular Devices RRID: SCR_011323; https://www. moleculardevices.com/ Spike2 Cambridge Electronic Design RRID: SCR_000903; https://ced.co.uk MATLAB The MathWorks, Inc., Natick, Massachusetts, United States. .. RRID: SCR_001622; https://www.mathworks. com ImageJ (Schneider et al., 2012) RRID: SCR_003070; https://imagej.nih.gov/ij/ Adobe Photoshop Adobe Systems Inc., San Jose, CA RRID:SCR_014199; https://www.adobe.com/ products/photoshop.html Adobe Illustrator Adobe Systems Inc., San Jose, CA RRID:SCR_010279; http://www.adobe.com/ products/illustrator.html (Continued on next page) Cell Reports 39, 110654, April 12, 2022 e1
Software:Article Title: Transformation of an early-established motor circuit during maturation in zebrafish.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-GFP Abcam Cat# ab13970, RRID:AB_300798 Rabbit anti-Cx35.5 clone 12H5 Fred Hutch Antibody Technology Facility (Miller et al., 2017) N/A Mouse IgG2A anti-Cx34.1 clone 5C10A Fred Hutch Antibody Technology Facility (Miller et al., 2017) N/A Secondary antibody anti-chicken Alexa Fluor 488 Invitrogen Cat# A11039, RRID:AB_2534096 Secondary antibody anti-rabbit Alexa Fluor 568 Invitrogen Cat# A10042, RRID:AB_2534017 Secondary antibody anti-mouse Alexa Fluor 647 Invitrogen Cat# A31571, RRID:AB_162542 Streptavidin, Alexa Fluor 647 conjugate Invitrogen Cat# S32357 Streptavidin, Alexa Fluor 405 conjugate Invitrogen Cat# S32351 Chemicals, peptides, and recombinant proteins Ethyl 3-aminobenzoate methanesulfonate (MS-222) Sigma-Aldrich Cat# E10521 Neurobiotin Vector Laboratories Cat# SP-1120 PBS – Phosphate-Buffered Saline (10X) pH 7.4 Invitrogen Cat# AM9625 Triton X-100 Sigma-Aldrich Cat# T9284 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Tetramethylrhodamine-dextran Thermo Fisher Cat# D3308 Alexa Fluor 647-dextran Thermo Fisher Cat# D22914 D-(-)-2-amino-5-phosphonoic acid (AP-5) Tocris Cat# 0106/1 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f] quinoxaline (NBQX) Tocris Cat# 1044/1 Cadmium Sigma-Aldrich Cat# 202908 Experimental models: Organisms/strains Danio rerio: Tg(Chx10:GFP) (Kimura et al., 2006) ZFIN: ZDB-GENO-010924-10 Danio rerio: Tg(Chx10-loxP-dsRed-loxP-GFP) (Kimura et al., 2006) ZFIN: ZDB-TGCONSTRCT-070117-143 Danio rerio: Tol-056:GFP (Satou et al., 2009) ZFIN: ZDB-FISH-150901-13314 Danio rerio: Tg(VGlut2a-loxP-dsRed-loxPGal4) (Satou et al., 2013) ZFIN: ZDB-TGCONSTRCT-131127-2 Danio rerio: Tg(elavl3:cre) (Förster et al., 2017) ZFIN: ZDB-TGCONSTRCT-170921-3 Danio rerio: Tg(UAS:ChR2-YFP) (Fidelin et al., 2015) ZFIN: ZDB-TGCONSTRCT-150324-2 Software and algorithms pClamp Molecular Devices RRID: SCR_011323; https://www. moleculardevices.com/ Spike2 Cambridge Electronic Design RRID: SCR_000903; https://ced.co.uk MATLAB The MathWorks, Inc., Natick, Massachusetts, United States. .. RRID: SCR_001622; https://www.mathworks. com ImageJ (Schneider et al., 2012) RRID: SCR_003070; https://imagej.nih.gov/ij/ Adobe Photoshop Adobe Systems Inc., San Jose, CA RRID:SCR_014199; https://www.adobe.com/ products/photoshop.html Adobe Illustrator Adobe Systems Inc., San Jose, CA RRID:SCR_010279; http://www.adobe.com/ products/illustrator.html (Continued on next page) Cell Reports 39, 110654, April 12, 2022 e1
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